Whether it is a headache or muscular pain, three of the major pain relievers are aspirin, acetaminophen, and ibuprofen. Analgesic drugs article about analgesic drugs by the free. January 24, 20 tlc analysis of analgesic drugs introduction. Chem 2 fall 2008 identification of over thecounter analgesics thinlayer chromatography tlc is a fast and simple method that can be used to identify organic molecules, verify the number of components in a mixture, or to monitor the progress of a reaction. Learning objectives understand the basic principles of polarity of organic compounds.
Analysis of analgesic, antipyretic, and nonsteroidal anti. Experimental procedure, report template introduction in this experiment, thin layer chromatography tlc will be used to examine the composition of various analgesic painrelieving drugs by comparison with the. If you use any reference, please, write the complete. Tlc key thinlayer chromatography lab complete answer key. Essay about tlc analysis of analgesic drugs 7 words. These types of analgesic drugs are taken for medical use in prescribed doses, where they act by relieving the pain and producing sleep. A total of 164 analgesic, antipyretic, and nonsteroidal antiinflammatory drugs were prescribed to 150 children aged 14 years 38. Almost all mixtures of solvents can be used as the mobile phase. The mixture is observed when it is in two different phase.
This absorbent is coated on a glass slide or plastic sheet creating a thin layer of the particular stationary phase. All of them should be written in paragraphs and the method should not be copied exactely from the instruction. Two examples of a solvent or the mobile phase that are mentioned in this experiment are 95% ethyl acetate and 5% acetic acid. Chromatographic are used extensively in organic chemistry laboratories for routines analysis. Lab report on tlc analisys of analgestic drugs essay examples 1266 words 6 pages tlc analysis of analgesic drugs introduction in this experiment, thinlayer chromatography tlc was used to determine the composition of various overthecounter otc analgesics.
Thin layer chromatography of the unknown analgesic free. Acetaminophen, acetylsalicylic acid, caffeine, and ibuprofen. These drugs also help in reducing fever and prevent platelet coagulation. Thin layer chromatography tlc is a solidliquid technique. Analysis of analgesics by thin layer chromatography. In this experiment, a thinlayer chromatography tlc was used to determine the composition of various overthecounter analgesics. Thin layer chromatography lab report freebooksummary. Solved experiment 3 tlc analysis of analgesic drugs. Thin layer chromatography analysis of analgesic drugs. Analysis of analgesics by thin layer chromatography ypen. You will be given five known reference substances, an unknown.
Thin layer chromatography in drug analysis thin layer chromatography in drug analysis chromatographic science series a series of textbooks and reference books editor. Thin layer chromatography, or tlc, is a method for analyzing mixtures by separating the. The analgesic contains insoluble binders so not all of it will dissolve. The antiblood clotting action is the reason why aspirin is used for the prevention of heart attacks. All of them should be written in paragraphs and the. Analgesic drugs definition of analgesic drugs by the free. Chromatography of analysis of analgesic drugs esther juarez. Analgesics act in various ways on the peripheral and central pain pathways and are regarded as one of the most valuable but equally dangerous groups of medications. Thin layer chromatography, or tlc, is a method for analyzing mixtures by separating the compounds in the mixture. The purpose of this lab was to determine the composition of four different overthecounter drugs anacin, bufferin, excedrin, and tylenol. These compositions were determined by using the method of thinlayer chromatography tlc of the four overthecounter drugs, which were then compared. The report should be up to 800 words and it should include. Using the known standards that were either synthesized or provided, identify the unknown analgesics in some overthecounter painkillers using tlc. Chromatography is a technique that we used to separate and identify individual components in a mixture.
This selectivity is an important distinction between an analgesic and an anesthetic. Analgesic drugs include the nonsteroidal antiinflammatory drugs nonsteroidal antiinflammatory drug, a drug that suppresses inflammation in a manner similar to steroids, but without the side effects of steroids. Introduction thin layer chromatography also known as tlc is the physical separation of a mixture into its individual components by distributing the components between a stationary phase the porous tlc plate and a mobile phase the solvent that moves through the stationary phase and carries the. Thin layer chromatography tlc is a technique which is sensitive, cheap and fast. Objective the purpose of this experiment is to identify an unknown proprietary drug using thinlayer chromatography.
Download to endnoterefman ris metadata show full item. The purpose of this experiment is to identify the brand name of an unknown commercial analgesic product by tlc analysis. Journal of pharmaceutical and biomedical analysis 2001, 24 56, 11691173. Draw a light pencil line about 1 cm from the end of each chromatographic plate. Analgesic drugs act in various ways on the peripheral and central nervous systems. Learn vocabulary, terms, and more with flashcards, games, and other study tools. In this experiment, thin layer chromatography tlc will be used to examine the composition of various analgesic painrelieving drugs by comparison with the samples of aspirin, acetaminophen and caffeine you obtained from previous experiments. Synthesize phenacetin, aspirin or acetaminophen using the following procedures. Worlds best powerpoint templates crystalgraphics offers more powerpoint templates than anyone else in the world, with over 4 million to choose from. Acetaminophen, acetylsalicylic acid, caffeine, and acetylsalicylic acid. Analgesic drugs synonyms, analgesic drugs pronunciation, analgesic drugs translation, english dictionary definition of analgesic drugs.
Medicinal plants also provide a rich source of analgesics. May 11, 2014 hi,this experiment is abouttlc analysis of analgesic drugsand i would like from you to write a report. Identification of overthecounter analgesics thinlayer chromatography tlc is a fast and simple method that can be used to identify organic molecules, verify the number of components in a mixture, or to monitor the progress of a reaction. Individual variability in pain perception and differences in the efficacy of analgesic drugs are complex phenomena and are partly genetically predetermined. Apr 03, 20 chromatography of analysis of analgesic drugs abstract.
The unknowns behavior in thinlayer chromatography will be compared with that of its possible component analgesics. Preface to the second edition modern pharmaceutical drug analysis essentially involves as a necessary integral component even greater horizons than the actual prevalent critical analysis of not only the active pharmaceutical substances but also the secondary pharmaceutical products i. Tlc analysis of analgesic drugs flashcards quizlet. Modern pharmaceutical drug analysis essentially involves as a necessary integral component even greater horizons than the actual prevalent critical analysis of not only the active pharmaceutical substances but also the secondary pharmaceutical products i. Analysis of drugs by thin layer chromatography experimental techniques required chromatography thinlayer t other documents. The other major classification of otc pain medication is nonsteroidal antiinflammatory drugs. The best known of these analgesics is aspirin, but several other chemically similar compounds are. Study guide for drug analysis examination drug analysis study guideversion 1. The doctor prescribed an analgesic for her aching muscles. Theyll give your presentations a professional, memorable appearance the kind of sophisticated look that.
Tlc can be used to help determine the number of components in a mixture, the identity of compounds, and the purity of a compound. Thinlayer chromatography of analgesics introduction in this experiment, you will use thin. That known analgesics will be used as well as an unknown, the determination of the experiment will be based of the exposure of the chemicals the analgesics may contain and will be seen under an uv light. Analgesic drugs definition of analgesic drugs by the. Be able to deduce the relative polarity of organic compounds by comparing the functional groups. Gas chromatographic analysis of drugs and pesticides. Lab report on tlc analisys of analgestic drugs essay. Thin layer chromatography analysis of analgesic drugs essay. Essentially, tlc has many purposes within the setting of lab. The chemical compositions of four analgesics anacin, excedrin, nuprin, and tylenol were determined by thin layer chromatography in this experiment using ethyl acetate as both the solvent and the elluent. Pain pharmacology and analgesia encyclopedia of life. Introduction today on the market, there are a variety of over the counter drugs that can be used to combat the aches and pains encountered in everyday living.
They are distinct from anesthetics, which temporarily affect, and in some instances completely eliminate, sensation. A tlc analysis might be summarized something like, using a silica. Antiinflammatory, analgesic and antipyretic drugs chapter 26. The purpose of this experiment is to use thin layer chromatography to identify analgesic in a mixture, to identify the unknown analgesic by comparing the r f value, to find and determine the r f for each analgesic. Data were analyzed in electronic databases and the variables were summarized by simple frequency. The development chamber is a small screwcapped jar with a 5. Analgesic, any drug that relieves pain selectively without blocking the conduction of nerve impulses, markedly altering sensory perception, or affecting consciousness. Pdf thin layer chromotography of analgesic drugs idayu. Essay about tlc analysis of analgesic drugs 7 words cram. In thin layer chromatography, a solid phase, the adsorbent, is coated onto a solid support as a thin layer usually on a glass, plastic, or aluminum support. The purpose of this experiment is to focus on the ingredients that the analgesic drugs may contain. Terms in this set 29 what is the purpose of tlc of analgesic drugs. Spot one plate with your 4 known standards acetaminophen, aspirin, caffeine, and ibuprofen and the other plate with the 5 unknown. In thinlayer chromatography, the stationary phase is a polar absorbent, usually finely ground alumina or silica particles.
Analgesic drugs article about analgesic drugs by the. A thin layer chromatograph plate is used to identify drug component under uv. In this experiment, you will use tlc to identify unknown analgesic painkillers using the table of analgesics and their components in the experimental section of this experiment. The possible unknowns and their analgesic ingredients will be anacin aspirin, caffeine, excedrin acetaminophen, caffeine, aspirin, motrin. That is quite a mouthful, so this group is commonly referred to by the acronym nsaids.
Thin layer chromatography in drug analysis docshare. As it was previously stated, through this lab, tlc was used in order to identify the substances that are present in analgesic drugs. Salicylic acid is the substance that has analgesic, antiinflammatory. South encyclopedia of life support systems eolss 8. Thin layer chromatography of the unknown analgesic jessica bajao, phoebe abalos, kevin antiga, carmelus aseneta 3biology 2 college of science, university of santo tomas, manila, philippines abstract the group used five different analgesics in this experiment. Analgesics include paracetamol known in north america as acetaminophen or simply apap, the nonsteroidal antiinflammatory drugs nsaids such as the salicylates, and opioid. Thin layer chromotography of analgesic drugs objectives. Nonnarcotic or nonopioid nsaid,s and aspirin like analgesics. Quantitative analysis of analgoantipyretics in dosage form using planar chromatography. Tlc analysis of analgesic drugs 3 separated, an organic solvent that moves up the plate as a capillary action, it could also be eluent, which is the process of removing analytes from the adsorbent by running a solvent. Rating is available when the video has been rented.
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